The lab says fixation will lead to the dyes to leak through the cells. I feel the idea is that fixation could destruction mobile membranes. I did locate a paper which describes fixation applying methanol or acetic acid, right after staining using a DCFDA spinoff. DCFDA could be the reagent https://zanderydjua.webdesign96.com/28486991/2-fdck-hcl-an-overview