The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR goods ended up digested and cloned into a pET21a+ vector applying NdeI and XhoI restriction enzymes. Some extent mutation was released by the fast adjust https://centrinone-b24680.tokka-blog.com/26736126/the-2-minute-rule-for-agx51